发表论文 [1] Zhen JZ, Wu H W*,Li XQ. High-level extracellular secretion and characterization of the thermophilic β-cyclodextrin glucanotranferase from Paenibacillus campinasensis in Escherichia coli, 3 Biotechnology (SCI,2019) [2] 郭彩娟,公杰,刘永杰,赵昌平,高世庆,吴华伟*,全基因组小麦 PPR 基因家族鉴定及表达分析,生物技术通报,2019,35(8):1-8 [3] Zhang XP, Ding BM, Wu HW, Wang JJ, Yang HL. A Label-free "Turn-on" Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA. Analytical Sciences, 2017, 33(2):165-169 (SCI) [4] Li YJ, Xiong T, Wu HW, Yang YZ. Visual DNA Microarray Coupled with Multiplex-PCR for the Rapid Detection of Twelve Genetically Modified Maize. Biochip Journal, 2016,10(1):42-47 (SCI) [5] 谌恩华,李相前,吴华伟*,β-环糊精葡萄糖基转移酶基因的克隆、表达及酶学性质,食品与发酵工业,2016,42(11):77-81 [6] 谌恩华,吴华伟*,李相前, 结晶纤维素的降解,草业科学,2016,33(11):2367-2374 [7] Wu H W, Yu X X, Chen L B,et al. cloning, overexpression and characterization of a thermostable pullulanase from Thermus thermophilus HB27,Protein Expression and Purification,2014,95:22-27 [8] 陈 希,王淑芬,王天赐,吴华伟*,产β-环糊精葡萄糖基础转移酶菌株的筛选、鉴定和酶学性质,湖北农业科学,2014, 53(8):1891-1894 [9] 吴华伟,蔚鑫鑫,陈雪秋,产甘露聚糖细菌的分离鉴定、酶的部分纯化及酶学性质研究,湖北农业科学,2014,53(15),3601-3605 [10] Le YL, Peng JJ, Wu HW, Sun JZ , Shao WL. An Approach to the Production of Soluble Protein from a Fungal Gene Encoding an Aggregation-Prone Xylanase in Escherichia coli. Plos One, 2011,6(4) (SCI) [11] 吴华伟,邵蔚蓝。Hsh载体对外源基因在E. coli中高效表达的机制探讨,微生物学通报 2011,38(9):1355-1361 [12] Wu H W, Pei J J, Shao W W, et al. pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes. Biotechnol Lett. 2010, 32:795-801 (SCI) [13] Shao W W, Wu H W, Pei J J. Novel expression vector system regulated by sigma32 and methods for using it to produce recombinant protein. Expression vector system regulated by and methods for using it to produce recombinant protein. 美国专利:Patent No.: US007807460B2, Oct. 5, 2010 [14] 吴华伟,张展,李相前. 易错PCR定向进化大幅度提高极耐热葡聚糖酶的活性,食品与发酵工业, 2010, 36() [15] 吴华伟,夏帆,李相前. 一种快速筛选重组耐高温-葡聚糖酶大肠杆菌的方法,食品与发酵工业,2009,35(7):44-48 [16] 吴华伟,邵蔚蓝,表达载体pHsh对大肠杆菌热休克系统中负调控机制的影响,中国生物工程杂志,2009,29(6):79-84 [17] Wu H W, Pei J J, Wu G G, Shao W W. Overexpression of GH10 endoxylanase XynB fromThermotoga maritima in Escherichia coli by a novel vector with potential for industrial application. Enzyme Microb Technol. 2008, 42:230–234. (SCI) |